Sirius can be employed according to the individual needs of each user. In its basic configuration the luminometer is ready for standard measurements. Sirius can be upgraded to form a powerful measurement system for any luminescence application.

 

The Sirius luminometer measures standard glow chemistry of single samples from a variety of sample formats. For flash chemistry, and to make operation more convenient, the instrument can be formatted with one or two built-in automatic reagent injectors. In stand-alone mode, the built-in microprocessor software supports four protocols for data evaluation, including background subtraction capabilities and replicate management, as well as printer output. Modular PC-Software further enhances performance by organizing the measurement dialog and data evaluation. The Sirius Luminometer combines a very small footprint with excellent performance and outstanding ease of use. The retractable drawer slides the sample into the measurement chamber. Due to the unique design, a variety of sample formats can be accommodated. It is not necessary to press any key to process the samples, in stand-alone or in PC-operation. Closing the sample drawer initiates measurement automatically.

 

Applications
Sirius ist suitable for all luminescence Flash and Glow Applications including
- Single and Dual Reporter Gene Assays
- Immunoassays
- ATP Assays
- DNA Probe Assays
- Phagocyte Function Assays
- Quantification of PCR Products

 

The Sirius Luminometer has been evaluated by the Research Department staff at Promega Corporation and has been certified for use with their Dual Luciferase "DLReady" (TM Promega Corp.) assay. 
 

Detector
Sirius Luminometer features advanced photoncounting electronics along with a highly sensitive detector. It provides the lowest signal background and highest linearity covering 6 decades. No cumbersome internal calibration is required and no gain switch is needed. Light collection is maximized through both the proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. As a result, reporter gene assays using firefly luciferase can now detect less than 1000 molecules of the enzyme with the Sirius Luminometer.

Injection System
Sirius is available with up to two high-precision reagent injectors. Variable injection volumes can be set from 20 to 300µl. Flexible speed settings and precise injector positioning allow homogenous
mixing of reagents for every type of sample. Short reagent lines and the back-pumping option
guarantee the most economical utilization of valuable reagents. Sirius is equipped with reliable,
bellow-type pumps. Selection of chemically inert materials assures the injectors are virtually
maintenance-free. Sirius will detect presence of a sample vial prior to injection, to avoid unintended
injection into an empty sample chamber. 

 

Stand-alone Operation
Sirius is equipped with on-board microprocessor software for instrument control, data calculation and printer output. In stand-alone mode Sirius supports four protocols designed to meet the needs of state of the art luminescence assays.

In addition to the Raw Data protocol, which allows standard measurements in a row, Single Assay,
Dual Assay and Cut-off Assay are available, supporting predefined measurement sequences, data evaluation and printer output.

 

Intuitive & Powerful PC Software
FB12/Sirius PC Software is designed for stationary use in the laboratory. In addition to the Quick Measurement function, it offers five additional protocol types: Single Assay, Dual Assay, Single Kinetics, Multiple Kinetics and Cut-off Assay. The operator can define new protocols of all five types. Highly flexible graphical presentation of kinetic data is supported.

Convenient data connection options
Sirius has both USB and serial ports.