Compact and affordable, powerful and reliable
The new Orion L Microplate Luminometer is a compact, very affordable and user-friendly instrument, which uses high quality materials and advanced technologies. The built-in USB port is an added feature allowing lab users to keep up with ever changing PC demands. This advanced luminometer will plug and play with any Windows PC. Very easy to maintain and upgradeable, it will be an asset to every laboratory. With the optional validation tools, verification of full functionality is performed automatically. Generic data format for result output ensure integrity of your original measurement data.

 

Reporter Gene Assays (Single and Dual Reading e.g. Dual Luciferase® Reporter Assay)

ATP Assays

Luminescent Immunoassays (LIA and ILMA)

Enzyme Assays (ATP or NAD(P)H)

Nucleid Acid Probe Assays

Aequorin-based

Ca++ Assays

Kinase Assays

Receptor Assays

SNP Genotyping (READIT®)

Dioxin contamination

Mycoplasma contamination (MycoAlert®)

…..and many more.

 

Orion L has been evaluated by the Research Department staff at Promega Corporation
and has been certified for use with their Dual Luciferase "DLReady" (TM Promega Corp.) assay. 
 

Sample format
The Orion L will accept any luminescence-grade microplates in either white or black. Highest sensitivity is obtained with white microplates. Recommendations for quality microplates are available on request, and the instrument is furnished with an initial supply of high quality microplates.

 

The Orion L is available with up to two high precision automatic reagent injectors. The injection profile is optimised for efficient and fast mixing of the reagents in the microplate well. The excellent match of all hydromechanical and electrical components in the injection system design lead to extremely accurate reproducibility of a flash reaction. All components of the injection system are easily accessible for maintenance, and do not require any special tools.

 

Simplicity – Automated Measurement Protocols

Simplicity is structured in the form of four protocols allowing automation of all steps from defining measurement parameters to reporting final results. By implementing different protocol types, the user can define customized protocols for immediate use or store them for later availability. The Simplicity protocol dialogue allows modifications of injection volume, delay time and measurement duration. Any combination of wells can be read. Simplicity allows Selection of the entire plate, or the functionality to specify random wells, rows or columns.

 

Simplicity – Protocol For Basic Applications

The basic module of Simplicity software consists of a standard Raw Data protocol. The measuring time can be set upwards from 0.1 s. The results are reported as RLU/s. The data is presented as a spreadsheet and may be printed upon completion of measurement or saved in different formats. In addition, Simplicity features automatic data transfer to predefined Microsoft Excel templates and Macros making data evaluation and presentation more flexible.

Fast Kinetics Protocol
Fast Kinetics protocol enables the user to monitor changes in luminescence per individual well. The total measuring time is pre-selected by the user. Duration of the measurement is divided into 50 data points, allowing a maximum time resolution of 2 ms. Measurement results are available as tables (kinetics and integral) and graphs. Graphical functions include: smoothing, averaging of selected samples, logarithmic scale, auto or selectable scale. Curve calculation functions include peak height (RLU/s and time), integral RLU/s of measurement, time of half maximum (rising) and time of half maximum (decay).

 

Dual Measurement Protocol
This protocol allows measuring of each well twice. It is typically used for dual reporter gene assays. Sequential injections and measurements can be easily implemented. For example, it may be programmed to perform the first measurement after injection of reagent one, followed by another injection with reagent two, and subsequent second measurement.

 

More than just an instrument – Lifetime service and support
Validation Tools
Our goal is to ensure that your instrument will be in excellent condition for the lifetime of the product. If there is a need to prove and document proper function, of the luminometer, Berthold Detection Systems offers a full line of quality validatation tools: The Luminescence TestPlate has stable light sources which span over the entire dynamic range of the luminometer. Packaged with our Intuitive and comprehensive software, the Luminescence TestPlatemakes automatic detector validation an easy task. Finally, our IQ/OQ/PQ documentation packages include all necessary instructions to test and document all instrument functions, meeting even the most demanding laboratory regulations (such as pharmaceutical R&D, and clinical product manufacturing).

 

Outstanding After Sales Support
Selling an instrument is only the beginning of the realtionshipfor us. Our experienced and customer-oriented staff is looking forward to help you with any inquiry, request for assistance or troubleshooting issue.

 

DLReady®, Dual Luciferase® READIT® and Stop & Glo® are trademarks of Promega Corporation. MycoAlert® is a trademark of Lonza. Excel® is a trademark of Microsoft Corporation.